AGL/Alpha-glucosidase Recombinant Rabbit Monoclonal Antibody [PSH04-56]
cat.: HA722155
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH04-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 175 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human AGL aa 783-1,532 / 1,532. |
| Positive control: | K-562 cell lysate, HEK-293 cell lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, human liver tissue, mouse liver tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P35573 Human Entrez Gene: 77559 Mouse | 362029 Rat |
| Alternative names: | 1110061O17Rik 4-1 4-glucantransferase 6-glucosidase 9430004C13Rik 9630046L06Rik AGL AI850929 Amylo 1 6 glucosidase 4 alpha glucanotransferase Amylo-1 Amylo-alpha-1 C77197 Dextrin 6-alpha-D-glucosidase GDE GDE_HUMAN Glycogen debrancher Glycogen debranching enzyme Glycogen storage disease type III Oligo-1 |
Images
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Fig1:
Western blot analysis of AGL/Alpha-glucosidase on different lysates with Rabbit anti-AGL/Alpha-glucosidase antibody (HA722155) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (20 µg/Lane) Lane 3: Mouse skeletal muscle tissue lysate (10 µg/Lane) Lane 4: Rat skeletal muscle tissue lysate (10 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 175 kDa Observed band size: 150 kDa Exposure time: Lane 1-2: 3 minutes; Lane 3-4: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722155) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-AGL/Alpha-glucosidase antibody (HA722155) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722155) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-AGL/Alpha-glucosidase antibody (HA722155) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722155) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.