Acid phosphatase/ACP1 Recombinant Rabbit Monoclonal Antibody [PSH04-57]
cat.: HA722156
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH04-57 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ACP1 aa 1-158 / 158. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, Jurkat cell lysate, COS-1 cell lysate, human kidney tissue, human stomach tissue, human testis tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P24666 Human |
| Alternative names: | Acid phosphatase 1 soluble Acid phosphatase of erythrocyte ACP1 Adipocyte acid phosphatase Cytoplasmic phosphotyrosyl protein phosphatase HAAP LMW-PTP LMW-PTPase Low molecular weight cytosolic acid phosphatase Low molecular weight phosphotyrosine protein phosphatase PAP1 PAP2 phosphatase, acid, of erythrocyte PPAC_HUMAN Protein tyrosine phosphatase PTPase Purple acid phosphatase Red cell acid phosphatase 1 testicular secretory protein Li 37 |
Images
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Fig1:
Western blot analysis of Acid phosphatase/ACP1 on different lysates with Rabbit anti-Acid phosphatase/ACP1 antibody (HA722156) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 1 minute 33 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722156) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Acid phosphatase/ACP1 antibody (HA722156) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Acid phosphatase/ACP1 antibody (HA722156) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Acid phosphatase/ACP1 antibody (HA722156) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722156) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.