SREBP1 Recombinant Rabbit Monoclonal Antibody [PSH04-61]
cat.: HA722160
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH04-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 122 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SREBP1 aa 301-350 / 1,147. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, MCF7 cell lysate, A549 cell lysate, SK-Br-3 cell lysate, human adrenal gland tissue, HeLa. |
| Subcellular location: | Endoplasmic reticulum membrane, Golgi apparatus membrane, Cytoplasmic vesicle, COPII-coated vesicle membrane; Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:2,000 1:1,000 1:100 |
| Uniprot #: | SwissProt: P36956 Human |
| Alternative names: | ADD 1 bHLHd1 Class D basic helix-loop-helix protein 1 D630008H06 Processed sterol regulatory element-binding protein 1 SRBP1_HUMAN SREBF 1 SREBF1 SREBP 1 SREBP 1c SREBP-1 SREBP1 Sterol regulatory element binding protein 1 Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 Sterol regulatory element binding transcription factor 1 Sterol regulatory element-binding transcription factor 1 |
Images
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Fig1:
Western blot analysis of SREBP1 on different lysates with Rabbit anti-SREBP1 antibody (HA722160) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: SK-Br-3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 122 kDa Observed band size: 80-100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722160) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of SREBP1 on different lysates with Rabbit anti-SREBP1 antibody (HA722160) at 1/2,000 dilution. Lane 1: HeLa-si-NT cell lysate Lane 2: HeLa-si-SREBP1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 122 kDa Observed band size: 100 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722160) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue with Rabbit anti-SREBP1 antibody (HA722160) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722160) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of HeLa cells labeling SREBP1 with Rabbit anti-SREBP1 antibody (HA722160) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SREBP1 antibody (HA722160) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.