DDIT4 Recombinant Rabbit Monoclonal Antibody [PS00-10]
cat.: HA722161
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PS00-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein. |
| Positive control: | HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate. |
| Subcellular location: | Mitochondrion, Cytoplasm, cytosol. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9NX09 Human |
| Alternative names: | DDIT4 DDIT4_HUMAN Dig2 DNA damage inducible transcript 4 DNA damage inducible transcript 4 protein DNA damage-inducible transcript 4 protein FLJ20500 HIF 1 responsive protein RTP801 HIF 1 responsive RTP801 HIF-1 responsive protein RTP801 Protein regulated in development and DNA damage response 1 REDD-1 REDD1 RTP801 |
Images
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Fig1:
Western blot analysis of DDIT4 on different lysates with Rabbit anti-DDIT4 antibody (HA722161) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 30 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722161) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.