DDIT4 Recombinant Rabbit Monoclonal Antibody [PS00-10]
cat.: HA722161
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: PS00-10
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 25 kDa
Isotype: IgG
Immunogen: Recombinant full length protein.
Positive control: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate.
Subcellular location: Mitochondrion, Cytoplasm, cytosol.
Recommended Dilutions:
  WB
1:1,000
Uniprot #: SwissProt: Q9NX09 Human
Alternative names: DDIT4 DDIT4_HUMAN Dig2 DNA damage inducible transcript 4 DNA damage inducible transcript 4 protein DNA damage-inducible transcript 4 protein FLJ20500 HIF 1 responsive protein RTP801 HIF 1 responsive RTP801 HIF-1 responsive protein RTP801 Protein regulated in development and DNA damage response 1 REDD-1 REDD1 RTP801
Images
HA722161_1.jpg Fig1: Western blot analysis of DDIT4 on different lysates with Rabbit anti-DDIT4 antibody (HA722161) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 25 kDa
Observed band size: 30 kDa

Exposure time: 50 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722161) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.