Human IL-5 Recombinant Rabbit Monoclonal Antibody [PSH04-64]
cat.: HA722164
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH04-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-5 aa 20-134 (P05113). |
| Positive control: | Recombinant Human IL-5 protein (HA210594). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-65] to Human IL-5 (Detector) (HA722165) and recombinant standard Human IL-5 (HA210594). The reference range value is 12.3-1000 pg/ml. |
| Uniprot #: | SwissProt: P05113 Human |
| Alternative names: | B-cell differentiation factor I Colony stimulating factor EDF Eosinophil differentiation factor IL-5 IL5 IL5_HUMAN Interleukin 5 (colony stimulating factor, eosinophil) Interleukin 5 Interleukin-5 T Cell Replacing Factor T-cell replacing factor TRF |
Images
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Fig1:
Sandwich ELISA analysis of Human IL-5 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722164) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-5 protein (HA210594) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA722165, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: Conditioned media was harvested after 6 days. IL-5 was measured in 100% unstimulated and PHA-M stimulated human PBMC cell supernatant. The concentrations of IL-5 were interpolated from the IL-5 standard curves. The mean IL-5 concentration was determined to be 475pg/mL in PHA-M stimulated human PBMC cell supernatant. There was no detectable signal in unstimulated supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.