LEFTY1+LEFTY2 Recombinant Rabbit Monoclonal Antibody [JE38-88]
cat.: HA722177
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE38-88 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human LEFTY2 aa 267-366 / 366. |
| Positive control: | A431 cell lysate, SiHa cell lysate, NCCIT cell lysate, HeLa cell lysate, ES-D3 cell lysate, F9 cell lysate, ES-W4 cell lysate, mouse heart tissue lysate, mouse lung tissue lysate, mouse embryo tissue lysate, rat heart tissue lysate, rat lung tissue lysate, rat embryo tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O75610 Human | O00292 Human | Q64280 Mouse | P57785 Mouse | D4A670 Rat Entrez Gene: 289316 Rat |
| Alternative names: | AI450052 Ebaf Left right determination factor 1 Left right determination factor Left right determination factor B left-right determination factor LEFT-RIGHT DETERMINATION FACTOR 1; LEFTY1 left-right determination, factor B LEFT-RIGHT DETERMINATION, FACTOR B; LEFTY B LEFTB LEFTY 1 Lefty lefty-1 LEFTY1 LEFTY1, MOUSE, HOMOLOG OF LEFTYB OTTHUMP00000035572 Protein lefty-1 Protein lefty-B RGD1561867 Stimulated by retinoic acid gene 3 protein STRA3 Endometrial bleeding associated factor (left right determination factor A transforming growth factor beta superfamily) Endometrial bleeding associated factor Endometrial bleeding-associated factor Left right determination factor 2 Left right determination factor A Left-right determination factor 2 Left-right determination factor A LEFTA LEFTY 2 LEFTY2 LEFTYA LFTY2 transforming growth factor, beta -4 LFTY2, mouse, homolog of LFTY2_HUMAN MGC46222 Protein lefty 2 Protein lefty...... |
Images
|
Fig1:
Western blot analysis of LEFTY1+LEFTY2 on different lysates with Rabbit anti-LEFTY1+LEFTY2 antibody (HA722177) at 1/1,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: SiHa cell lysate (15 µg/Lane) Lane 3: NCCIT cell lysate (15 µg/Lane) Lane 4: HeLa cell lysate (15 µg/Lane) Lane 5: ES-D3 cell lysate (15 µg/Lane) Lane 6: F9 cell lysate (15 µg/Lane) Lane 7: ES-W4 cell lysate (15 µg/Lane) Lane 8: Mouse heart tissue lysate (30 µg/Lane) Lane 9: Mouse lung tissue lysate (30 µg/Lane) Lane 10: Mouse embryo tissue lysate (30 µg/Lane) Lane 11: Rat heart tissue lysate (30 µg/Lane) Lane 12: Rat lung tissue lysate (30 µg/Lane) Lane 13: Rat embryo tissue lysate (30 µg/Lane) Predicted band size: 41 kDa Observed band size: 28-41 kDa Exposure time: 1 minute ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722177) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.