Phospho-ErbB3/HER3 (Y1289) Recombinant Rabbit Monoclonal Antibody [JE51-25]
cat.: HA722178
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE51-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 148 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to a region surrounding Tyr1289 of Human ErbB3. |
| Positive control: | MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, MCF7 cells serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes. |
| Subcellular location: | Cell membrane, secreted. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: P21860 Human |
| Alternative names: | c erbB 3 c erbB3 Erb b2 receptor tyrosine kinase 3 ErbB 3 ERBB3 ERBB3 protein erbB3 S ERBB3_HUMAN Glial growth factor receptor HER 3 HER3 Human epidermal growth factor receptor 3 LCCS2 MDA BF 1 MGC88033 p180 ErbB3 p45 sErbB3 p85 sErbB3 proto-oncogene-like protein c ErbB 3 proto-oncogene-like protein c ErbB3 Proto-oncogene-like protein c-ErbB-3 Receptor tyrosine protein kinase erbB 3 Receptor tyrosine protein kinase erbB3 Receptor tyrosine-protein kinase erbB-3 Tyrosine kinase type cell surface receptor HER3 Tyrosine kinase-type cell surface receptor HER3 v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) v erb b2 erythroblastic leukemia viral oncogene homolog 3 |
Images
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Fig1:
Western blot analysis of Phospho-ErbB3/HER3 (Y1289) on different lysates with Rabbit anti-Phospho-ErbB3/HER3 (Y1289) antibody (HA722178) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate Lane 3: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 148 kDa Observed band size: 180 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722178) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of MCF7 cells serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes labeling Phospho-ErbB3/HER3 (Y1289) with Rabbit anti-Phospho-ErbB3/HER3 (Y1289) antibody (HA722178) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-ErbB3/HER3 (Y1289) antibody (HA722178) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.