MyoD1 Recombinant Rabbit Monoclonal Antibody [JE60-47]
cat.: HA722179
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: JE60-47
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 35 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human MyoD1 aa 1-100 / 320.
Positive control: RD cell lysate, RD.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell

1:1,000
1:100
Uniprot #: SwissProt: P15172 Human
Alternative names: bHLHc1 Class C basic helix-loop-helix protein 1 MYF 3 Myf-3 MYF3 Myoblast determination protein 1 Myod 1 MYOD MYOD1 MYOD1_HUMAN Myogenic differentiation 1 Myogenic factor 3 Myogenic factor MYF 3 Myogenin D1 PUM
Images
HA722179_1.jpg Fig1: Western blot analysis of MyoD1 on different lysates with Rabbit anti-MyoD1 antibody (HA722179) at 1/1,000 dilution.

Lane 1: RD cell lysate
Lane 2: HEK-293 cell lysate (negative)
Lane 3: HeLa cell lysate (negative)
Lane 4: RD cell lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 45 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722179) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722179_2.jpg Fig2: Immunocytochemistry analysis of RD (positive) and HEK-293 (negative) labeling MyoD1 with Rabbit anti-MyoD1 antibody (HA722179) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MyoD1 antibody (HA722179) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.