RIP3 Recombinant Rabbit Monoclonal Antibody [PSH04-78]
cat.: HA722182
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH04-78 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human RIP3 aa 401-450 / 518. |
| Positive control: | THP-1 cell lysate, HT-29 cell lysate, SK-Br-3 cell lysate, HT-29, human colon cancer tissue. |
| Subcellular location: | Cytoplasm, cytosol, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:2,000-1:5,000 |
| Uniprot #: | SwissProt: Q9Y572 Human |
| Alternative names: | Receptor interacting protein 3 Receptor interacting serine threonine kinase 3 Receptor interacting serine/threonine protein kinase 3 Receptor-interacting protein 3 Receptor-interacting serine/threonine-protein kinase 3 RIP 3 RIP like protein kinase 3 RIP-3 RIP-like protein kinase 3 RIPK 3 RIPK3 RIPK3_HUMAN |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded HT-29 (positive) and HeLa (negative) cells with Rabbit anti-RIP3 antibody (HA722182) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722182) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-RIP3 antibody (HA722182) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722182) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Western blot analysis of RIP3 on different lysates with Rabbit anti-RIP3 antibody (HA722182) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HT-29 cell lysate Lane 3: SK-Br-3 cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 30 µg/Lane. Exposure time: 1 minutes; ECL: K1801 HeLa is a negative control. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA722182, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 57 kDa Observed band size: 57 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.