HA722186

PMCA1 Recombinant Rabbit Monoclonal Antibody [JE34-05]

cat.: HA722186

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P
Clonality:	Monoclonal
Clone number:	JE34-05

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 135 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human PMCA1 aa 1,081-1,180 / 1,220.
Positive control:	A549 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human lung tissue, human stomach tissue, human brain tissue, mouse brain tissue, rat brain tissue.
Subcellular location:	Cell membrane, Basolateral cell membrane, Synapse, Presynaptic cell membrane, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.
Recommended Dilutions: WB IHC-P	1:1,000-1:5,000 1:200-1:2,000
Uniprot #:	SwissProt: P20020 Human \| G5E829 Mouse \| P11505 Rat
Alternative names:	AT2B1_HUMAN ATP2B1 ATPase Ca++ transporting plasma membrane 1 Plasma membrane calcium ATPase 1 Plasma membrane calcium ATPase isoform 1 Plasma membrane calcium pump isoform 1 Plasma membrane calcium transporting ATPase 1 Plasma membrane calcium-transporting ATPase 1 PMCA 1 PMCA1

Images

Fig1: Western blot analysis of PMCA1 on different lysates with Rabbit anti-PMCA1 antibody (HA722186) at 1/1,000 dilution.

Lane 1: A549 cell lysate (no heat)
Lane 2: A549 cell lysate

Notice: no heat means the lysate is not boiled.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 135 kDa
Observed band size: 135-250 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722186) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of PMCA1 on different lysates with Rabbit anti-PMCA1 antibody (HA722186) at 1/5,000 dilution.

Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Rat brain tissue lysate (no heat)

Notice: no heat means the lysate is not boiled.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 135 kDa
Observed band size: 135 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722186) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PMCA1 antibody (HA722186) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722186) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-PMCA1 antibody (HA722186) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722186) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PMCA1 antibody (HA722186) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722186) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PMCA1 antibody (HA722186) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722186) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PMCA1 antibody (HA722186) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722186) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.