Human CD30 Recombinant Rabbit Monoclonal Antibody [PSH04-82]
cat.: HA722188
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH04-82 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD30 aa 19-385 (P28908). |
| Subcellular location: | Cell membrane; Cytoplasm. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-81] to Human CD30 (Capture) (HA722187) and recombinant standard Human CD30 protein (HA210649). The reference range value is 31-2500pg/ml. |
| Uniprot #: | SwissProt: P28908 Human |
| Alternative names: | CD 30 CD30 CD30 antigen CD30L receptor Cytokine receptor CD30 D1S166E KI 1 KI 1 antigen Ki-1 antigen KI1 Lymphocyte activation antigen CD30 TNFRSF 8 Tnfrsf8 TNR8_HUMAN Tumor necrosis factor receptor superfamily member 8 |
Images
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Fig1:
Sandwich ELISA analysis of human CD30 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722187) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD30 protein (HA210649) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722188) (Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: The concentrations of CD30 were interpolated from the CD30 standard curves and corrected for sample dilution. Undiluted samples are HDLM-2 cell culture supernatant 50%. The mean CD30 concentration was determined to be 1,260 pg/ml in HDLM-2 cell culture supernatant. |
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Fig3: The concentrations of CD30 were interpolated from the CD30 standard curves and corrected for sample dilution. Undiluted samples are mixed serum from twenty-four volunteers 13%. The mean CD30 concentration was determined to be 11,531 pg/ml in mixed serum from twenty-four volunteers. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.