Human MBL2 Recombinant Rabbit Monoclonal Antibody [PSH04-85]
cat.: HA722191
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH04-85 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MBL2 aa 108-248 (P11226). |
| Positive control: | Recombinant Human MBL2 protein (HA210628). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-86] to Human MBL2 (Detector) (HA722192) and recombinant standard Human MBL2 protein (HA210628). The reference range value is 99-8000 pg/ml. |
| Uniprot #: | SwissProt: P11226 Human |
| Alternative names: | COLEC 1 COLEC1 Collectin-1 HSMBPC Lectin, mannose-binding, soluble, 2 Mannan binding lectin Mannan binding protein Mannan-binding protein Mannose binding lectin (protein C) 2 soluble Mannose binding lectin (protein C) 2, soluble (opsonic defect) Mannose binding lectin (protein C) 2, soluble Mannose binding lectin 2 soluble Mannose binding lectin 2, soluble (opsonic defect) Mannose binding lectin Mannose binding lectin protein C2 soluble opsonic defect Mannose binding protein Mannose binding protein C Mannose binding protein C precursor Mannose binding protein, serum Mannose-binding lectin Mannose-binding protein C MBL 2 MBL MBL2 MBL2_HUMAN MBL2D MBP 1 MBP MBP C MBP-C MBP1 MBPB MBPC MBPD MGC116832 MGC116833 Opsonic defect protein C Soluble mannose binding lectin |
Images
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Fig1:
Sandwich ELISA analysis of Human MBL2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722191) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human MBL2 protein (HA210628) starting from 8000 pg/ml to 0 pg/ml and detect antibody (HA722192, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2: The concentrations of MBL2 were interpolated from the MBL2 standard curves and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 100%. The mean MBL2 concentration was determined to be 1,936 pg/ml in HepG2 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.