Mouse IL12 p40 Recombinant Rabbit Monoclonal Antibody [PSH04-87]
cat.: HA722193
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH04-87 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse IL-12B (aa 23-335)-GSGSSRGGSGSGGSGGGGSK-IL-12A (aa 23-215). |
| Positive control: | Recombinant mouse IL-12p40 protein (HA210890). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-88] to Mouse IL-12p40 (Detector) (HA722194) and recombinant standard Mouse IL-12p40 protein (HA210890). The reference range value is 37-3000pg/ml. |
| Uniprot #: | SwissProt: P43432 Mouse |
| Alternative names: | CLMF CLMF p40 CLMF2 Cytotoxic lymphocyte maturation factor 40 kDa subunit IL 12 subunit p40 IL 12B IL-12 subunit p40 IL-12B IL12 p40 IL12 subunit p40 IL12B IL12B_HUMAN interleukin 12 beta chain Interleukin 12 p40 Interleukin 12 subunit beta Interleukin 12B Interleukin-12 subunit beta natural killer cell stimulatory factor 40 kD subunit NK cell stimulatory factor chain 2 NKSF NKSF2 p40 |
Images
|
Fig1:
Sandwich ELISA analysis of Mouse IL-12p40 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722193) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant mouse IL-12p40 protein (HA210890) starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA722194, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native IL-12 p40 in cell culture supernatant and mouse serum samples. IL-12 p40 was measured in 100% cell supernatant. The concentrations of IL-12 p40 were interpolated from the IL-12 p40 standard curves. The mean IL-12 p40 concentration was determined to be 4055 pg/mL in mouse serum. There was no detectable signal in NIH/3T3 cell supernatant. |
|
Fig3:
Interpolated concentrations of spiked IL-12 p40 in mouse cell culture media samples. The concentrations of IL-12 p40 were measured in duplicates, interpolated from the IL-12 p40 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. |
|
Fig4:
Interpolated concentrations of native IL-12 p40 in mouse serum sample. The concentrations of IL-12 p40 were interpolated from the IL-12 p40 standard curves and corrected for sample dilution. Undiluted samples are serum 100%. The mean IL-12 p40 concentration was determined to be 4055 pg/mL in mouse serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.