TPH2 Recombinant Rabbit Monoclonal Antibody [PSH04-89]
cat.: HA722195
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH04-89 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein with Human THP2 aa 1-490. |
| Positive control: | Mouse hippocampus tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, mouse midbrain tissue, mouse cerebrum tissue, rat cerebrum tissue, mouse brain (raphe nucleus) tissue. |
| Subcellular location: | Cytosol, neuron projection. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IP |
1:2,000 1:500 1:500 1-2μg/sample |
| Uniprot #: | SwissProt: Q8CGV2 Mouse | Q8CGU9 Rat |
| Alternative names: | MGC138871 ADHD7 FLJ37295 MGC138872 Neuronal tryptophan hydroxylase NTPH TPH 2 Tph2 TPH2_HUMAN Tryptophan 5-hydroxylase 2 Tryptophan 5-monooxygenase 2 Tryptophan hydroxylase 2 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Raphe nucleus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse midbrain tissue with Rabbit anti-TPH2 antibody (HA722195) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722195) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-TPH2 antibody (HA722195) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722195) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-TPH2 antibody (HA722195) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722195) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of TPH2 on different lysates with Rabbit anti-TPH2 antibody (HA722195) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse kidney tissue lysate (negative) Lane 3: Rat brain tissue lysate Lane 4: Rat kidney tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722195) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig6:
TPH2 was immunoprecipitated from 0.2 mg rat brain tissue lysate with HA722195 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722195 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: rat brain tissue lysate (input) Lane 2: HA722195 IP in rat brain tissue lysate Lane 3: Rabbit IgG instead of HA722195 in rat brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.