AKR1C3 Recombinant Rabbit Monoclonal Antibody [JE36-28]
cat.: HA722198
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE36-28 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human AKR1C3 aa 181-280 / 323. |
| Positive control: | A549 cell lysate, HepG2 cell lysate, K-562 cell lysate, human kidney tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P42330 Human Entrez Gene: 171516 Rat |
| Alternative names: | 17 beta HSD 5 17 beta hydroxysteroid dehydrogenase type 5 17-beta-HSD 5 17-beta-hydroxysteroid dehydrogenase type 5 2-dihydrobenzene-1 2-diol dehydrogenase 20 alpha-hydroxysteroid dehydrogenase 20-alpha-hydroxysteroid dehydrogenase 3 alpha hydroxysteroid dehydrogenase type II 3-alpha-HSD type 2 3-alpha-HSD type II 3-alpha-HSD type II, brain 3-alpha-hydroxysteroid dehydrogenase type 2 AK1C3_HUMAN AKR1 C3 Akr1c18 AKR1C3 Aldo keto reductase family 1 member C3 Aldo-keto reductase family 1 member C3 brain Chlordecone reductase Chlordecone reductase homolog HAKRb DD-3 DD3 DDH1 DDX Dihydrodiol dehydrogenase 3 Dihydrodiol dehydrogenase type I Dihydrodiol dehydrogenase X HA1753 HAKRB HAKRe hluPGFS HSD17B5 Indanol dehydrogenase KIAA0119 PGFS Prostaglandin F synthase Testosterone 17-beta-dehydrogenase 5 Trans-1 Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase Type IIb 3 alpha hydroxysteroid dehydrogenase |
Images
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Fig1:
Western blot analysis of AKR1C3 on different lysates with Rabbit anti-AKR1C3 antibody (HA722198) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: Human kidney tissue lysate (40 µg/Lane) Lane 5: Mouse kidney tissue lysate (40 µg/Lane) Lane 6: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 37 kDa Observed band size: 35 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722198) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.