GRP78 / BIP Recombinant Rabbit Monoclonal Antibody [JE01-35]
cat.: HA722202
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE01-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 72 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GRP78 / BIP aa 1-100 / 654. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, A549 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, human breast cancer tissue, human brain tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Endoplasmic reticulum lumen, Melanosome, Cytoplasm, Cell surface. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:1,000 1:200 |
| Uniprot #: | SwissProt: P11021 Human | P20029 Mouse | P06761 Rat |
| Alternative names: | 78 kDa glucose regulated protein 78 kDa glucose-regulated protein AL022860 AU019543 BIP D2Wsu141e D2Wsu17e Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Endoplasmic reticulum lumenal Ca2+ binding protein grp78 Epididymis secretory sperm binding protein Li 89n FLJ26106 Glucose Regulated Protein 78 Glucose Regulated Protein 78kDa GRP 78 GRP-78 GRP78 GRP78_HUMAN Heat shock 70 kDa protein 5 Heat Shock 70kDa Protein 5 Heat shock protein family A (Hsp70) member 5 HEL S 89n Hsce70 HSPA 5 HSPA5 Immunoglobulin Heavy Chain Binding Protein Immunoglobulin heavy chain-binding protein mBiP MIF2 Sez7 |
Images
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Fig1:
Western blot analysis of GRP78 / BIP on different lysates with Rabbit anti-GRP78 / BIP antibody (HA722202) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: MDA-MB-231 cell lysate (20 µg/Lane) Lane 5: A549 cell lysate (20 µg/Lane) Lane 6: Mouse liver tissue lysate (40 µg/Lane) Lane 7: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: 1 minute 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722202) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-GRP78 / BIP antibody (HA722202) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722202) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GRP78 / BIP antibody (HA722202) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722202) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GRP78 / BIP antibody (HA722202) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722202) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GRP78 / BIP antibody (HA722202) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722202) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.