ApoER2 Recombinant Rabbit Monoclonal Antibody [JE31-73]
cat.: HA722203
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE31-73 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 106 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ApoER2 aa 864-963 / 963. |
| Positive control: | SH-SY5Y cell lysate, A549 cell lysate, U-87 MG cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, C6 cell lysate, human testis tissue. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q14114 Human | Q924X6 Mouse Entrez Gene: 362558 Rat |
| Alternative names: | APOER2 Apolipoprotein E receptor 2 low density lipoprotein receptor-related protein 8 Low-density lipoprotein receptor-related protein 8 LRP-8 LRP8 LRP8_HUMAN |
Images
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Fig1:
Western blot analysis of ApoER2 on different lysates with Rabbit anti-ApoER2 antibody (HA722203) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: A549 cell lysate Lane 3: U-87 MG cell lysate Lane 4: HepG2 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 106 kDa Observed band size: 100-150 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722203) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ApoER2 on different lysates with Rabbit anti-ApoER2 antibody (HA722203) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ApoER2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 106 kDa Observed band size: 100-150 kDa Exposure time: 2 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722203) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ApoER2 antibody (HA722203) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722203) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.