Glucokinase Recombinant Rabbit Monoclonal Antibody [JE33-40]
cat.: HA722204
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE33-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Glucokinase aa 216-465 / 465. |
| Positive control: | SiHa cell lysate, BxPC-3 cell lysate, HeLa cell lysate, Huh7 cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus, Mitochondrion. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P35557 Human |
| Alternative names: | ATP:D-hexose 6-phosphotransferase FGQTL3 GCK GK GLK Glucokinase Hexokinase D pancreatic isozyme Hexokinase type IV Hexokinase-4 Hexokinase-D HHF3 HK IV HK4 HKIV HXK4_HUMAN HXKP LGLK MODY2 |
Images
|
Fig1:
Western blot analysis of Glucokinase on different lysates with Rabbit anti-Glucokinase antibody (HA722204) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: BxPC-3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722204) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Glucokinase on different lysates with Rabbit anti-Glucokinase antibody (HA722204) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Huh7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 10 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722204) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.