ZBTB7A Recombinant Rabbit Monoclonal Antibody [JE34-74]
cat.: HA722205
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE34-74 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ZBTB7A aa 500 to the C-terminus. |
| Positive control: | MCF7 cell lysate, HepG2 cell lysate, K-562 cell lysate, HeLa cell lysate, HeLa, human tonsil tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P IP |
1:1,000 1:1,000 1:200-1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: O95365 Human |
| Alternative names: | DKFZp547O146 Factor binding IST protein 1 Factor that binds to inducer of short transcripts protein 1 FBI-1 FBI1 HIV-1 1st-binding protein 1 HIV-1 inducer of short transcripts binding protein HIV-1 inducer of short transcripts-binding factor 1 Leukemia/lymphoma-related factor LRF lymphoma related factor MGC99631 POK erythroid myeloid ontogenic factor Pokemon POZ and Krueppel erythroid myeloid ontogenic factor TIP21 TTF-I-interacting peptide 21 ZBT7A_HUMAN ZBTB7 ZBTB7A Zinc finger and BTB domain containing 7A zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein Zinc finger and BTB domain-containing protein 7A Zinc finger protein 857A Zinc finger- and BTB domain-containing protein 7 ZNF857A |
Images
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Fig1:
Western blot analysis of ZBTB7A on different lysates with Rabbit anti-ZBTB7A antibody (HA722205) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: K-562 cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722205) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling ZBTB7A with Rabbit anti-ZBTB7A antibody (HA722205) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZBTB7A antibody (HA722205) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-ZBTB7A antibody (HA722205) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722205) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
ZBTB7A was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722205 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722205 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA722205 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA722205 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.