Mouse PD-L1 Recombinant Rabbit Monoclonal Antibody [PSH05-04]
cat.: HA722209
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | FC |
| Clonality: | Monoclonal |
| Clone number: | PSH05-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse PD-L1 aa 19-239 / 290. |
| Positive control: | RAW264.7 cells treated with 10μg/mL LPS for 8 hours. |
| Subcellular location: | Cell membrane, Early endosome membrane, Recycling endosome membrane. |
| Recommended Dilutions:
FC |
1:1,000 |
| Uniprot #: | SwissProt: Q9EP73 Mouse |
| Alternative names: | B7 H B7 H1 B7 homolog 1 B7-H1 B7H B7H1 CD 274 CD-274 CD274 CD274 antigen CD274 molecule MGC142294 MGC142296 OTTHUMP00000021029 PD L1 PD-L1 PD1L1_HUMAN PDCD1 ligand 1 PDCD1L1 PDCD1LG1 PDL 1 PDL1 Programmed cell death 1 ligand 1 Programmed death ligand 1 RGD1566211 |
Images
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Fig1:
Flow cytometric analysis of RAW264.7 cells treated with 10μg/mL LPS for 8 hours labeling Mouse PD-L1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722209, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.