Human CD5 Recombinant Rabbit Monoclonal Antibody [PSH05-01]
cat.: HA722210
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IF-Cell, FC
Clonality: Monoclonal
Clone number: PSH05-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 55 kDa
Isotype: IgG
Immunogen: Recombinant protein within human CD5 aa 25-372 / 495.
Positive control: Jurkat, human peripheral blood lymphocytes
Subcellular location: Cell membrane.
Recommended Dilutions:
  IF-Cell
  FC

1:100
1:1,000
Uniprot #: SwissProt: P06127 Human
Alternative names: CD 5 CD5 CD5 antigen (p56 62) CD5 antigen CD5 molecule CD5_HUMAN LEU1 LyA Lymphocyte antigen T1/Leu-1 OTTHUMP00000236973 T-cell surface glycoprotein CD5 T1
Images
HA722210_1.jpg Fig1: Immunocytochemistry analysis of Jurkat (positive) and 293T (negative) labeling Human CD5 with Rabbit anti-Human CD5 antibody (HA722210) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Human CD5 antibody (HA722210) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA722210_2.jpg Fig2: Flow cytometric analysis of human peripheral blood lymphocytes labeling Human CD5.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722210, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.