Human CD200R1 Recombinant Rabbit Monoclonal Antibody [PSH05-02]
cat.: HA722211
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH05-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD200R1 aa 29-265 / 348. |
| Positive control: | Human peripheral blood. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IF-Cell FC |
1:100 1:1,000 |
| Uniprot #: | SwissProt: Q8TD46 Human |
| Alternative names: | CD 200R CD200 cell surface glycoprotein receptor CD200 receptor 1 Cd200r1 Cell surface glycoprotein CD200 receptor 1 Cell surface glycoprotein OX2 receptor 1 Cell surface glycoprotein OX2 receptor cell surface glycoprotein receptor CD200 CRTR 2 CRTR2 HCRTR2 MO2R1_HUMAN MOX2 receptor MOX2R OX2R |
Images
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Fig1:
Immunocytochemistry analysis of HeLa cells labeling Human CD200R1 with Rabbit anti-Human CD200R1 antibody (HA722211) at 1/100 dilution. HeLa cells, transfected with empty control, CD200R1 or CD200R2 expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Human CD200R1 antibody (HA722211) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig2:
Flow cytometric analysis of human peripheral blood labeling Human CD200R1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722211, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.