VAV3 Recombinant Rabbit Monoclonal Antibody [JE33-02]
cat.: HA722212
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE33-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 98 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human VAV3 aa 748-847 / 847. |
| Positive control: | Jurkat cell lysate, HeLa cell lysate, MCF7 cell lysate, rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, immunological synapse, plasma membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9UKW4 Human Entrez Gene: 295378 Rat |
| Alternative names: | FLJ40431 Guanine nucleotide exchange factor VAV3 Protein vav 3 Protein vav3 RGD1565941 VAV 3 Vav 3 guanine nucleotide exchange factor VAV 3 oncogene VAV 3 protein VAV-3 Vav3 VAV3 oncogene VAV3 protein VAV3_HUMAN |
Images
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Fig1:
Western blot analysis of VAV3 on different lysates with Rabbit anti-VAV3 antibody (HA722212) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (low expression) (20 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 98 kDa Observed band size: 98 kDa Exposure time: 46 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722212) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.