GGT1 Recombinant Rabbit Monoclonal Antibody [JE38-79]
cat.: HA722214
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE38-79 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GGT1 aa 1-100 / 569. |
| Positive control: | HepG2 cell lysate, A549 cell lysate, human liver tissue lysate, human kidney tissue lysate, human kidney tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P19440 Human |
| Alternative names: | CD224 D22S672 D22S732 Gamma glutamyl transpeptidase Gamma glutamyltransferase 1 Gamma glutamyltranspeptidase 1 Gamma-glutamyltransferase 1 Gamma-glutamyltranspeptidase 1 light chain GGT 1 GGT GGT1 GGT1_HUMAN Glutamyl transpeptidase Glutathione hydrolase 1 GTG Leukotriene C4 hydrolase MGC96892 MGC96904 MGC96963 OTTHUMP00000028921 OTTHUMP00000197959 |
Images
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Fig1:
Western blot analysis of GGT1 on different lysates with Rabbit anti-GGT1 antibody (HA722214) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: Human liver tissue lysate (40 µg/Lane) Lane 4: Human kidney tissue lysate (40 µg/Lane) Predicted band size: 61 kDa Observed band size: 60-70 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722214) at 1/1,000 dilution was used in antibody diluent at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GGT1 antibody (HA722214) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722214) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.