Cleaved PARP1 Recombinant Rabbit Monoclonal Antibody [JE00-15]
cat.: HA722218
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE00-15 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PARP1 aa 164-213. |
| Positive control: | Jurkat cell lysate, Jurkat treated with 1μM staurosporine for 3 hours cell lysate, HeLa cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, COS-1 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P09874 Human | P11103 Mouse | P27008 Rat |
| Alternative names: | ADP ribosyltransferase diphtheria toxin like 1 ADP ribosyltransferase NAD(+) ADP-ribosyltransferase diphtheria toxin-like 1 ADPRT 1 ADPRT ADPRT1 APOPAIN ARTD1 NAD(+) ADP-ribosyltransferase 1 PARP PARP-1 PARP1 PARP1_HUMAN Poly [ADP-ribose] polymerase 1 (PARP-1) Poly [ADP-ribose] polymerase 1 Poly ADP ribose polymerase 1 Poly(ADP ribose) polymerase Poly[ADP-ribose] synthase 1 PPOL SCA1 |
Images
|
Fig1:
Western blot analysis of Cleaved PARP1 on different lysates with Rabbit anti-Cleaved PARP1 antibody (HA722218) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Jurkat treated with 1μM staurosporine for 3 hours cell lysate Lane 3: HeLa cell lysate Lane 4: Neuro-2a cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722218) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.