NSUN2 Recombinant Rabbit Monoclonal Antibody [PSH05-05]
cat.: HA722219
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Monkey |
| Applications: | WB, IF-Cell, IHC-P, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH05-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NSUN2 aa1-767. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, 293T cell lysate, A431 cell lysate, HCT 116 cell lysate, Caco-2 cell lysate, Neuro-2a cell lysate, COS-1 cell lysate, human testis tissue, human small intestine tissue, HeLa. |
| Subcellular location: | Nucleus, nucleolus, Cytoplasm, Mitochondrion, cytoskeleton, spindle, Secreted, extracellular exosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P IF-Tissue IP |
1:2,000 1:250 1:1,000 1:200 1-2μg/sample |
| Uniprot #: | SwissProt: Q08J23 Human | Q1HFZ0 Mouse Entrez Gene: 361191 Rat |
| Alternative names: | 5 methycytoisine methyltransferase D13Wsu123e FLJ20303 hTrm4 MISU Myc induced SUN domain containing protein NOL1/NOP2/Sun domain family 2 NOL1/NOP2/Sun domain family 2 protein NOL1/NOP2/Sun domain family member 2 NSUN 2 NSUN2 NSUN2_HUMAN SAKI Substrate of AIM1/Aurora kinase B TRM4 tRNA (cytosine 5 ) methyltransferase tRNA (cytosine 5 ) methyltransferase NSUN2 tRNA (cytosine(34)-C(5))-methyltransferase tRNA (cytosine-5-)-methyltransferase tRNA methyltransferase 4 homolo tRNA methyltransferase 4 homolog |
Images
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Fig1:
Western blot analysis of NSUN2 on different lysates with Rabbit anti-NSUN2 antibody (HA722219) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: Jurkat cell lysate Lane 4: HEK-293 cell lysate Lane 5: 293T cell lysate Lane 6: A431 cell lysate Lane 7: HCT 116 cell lysate Lane 8: Caco-2 cell lysate Lane 9: Neuro-2a cell lysate Lane 10: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722219) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NSUN2 antibody (HA722219) at 1/1,000 dilution and competitor's antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722219) at 1/1,000 dilution and competitor's antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-NSUN2 antibody (HA722219) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722219) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of HeLa cells labeling NSUN2 with Rabbit anti-NSUN2 antibody (HA722219) at 1/250 dilution and competitor's antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NSUN2 antibody (HA722219) at 1/250 dilution and competitor's antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
NSUN2 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722219 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722219 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA722219 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA722219 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL: K1801 |
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