RNase L Recombinant Rabbit Monoclonal Antibody [JE36-11]
cat.: HA722221
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE36-11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RNase L aa 492-741 / 741. |
| Positive control: | THP-1 cell lysate, THP-1 treated with 20ng/mL IFN-γ for 48 hours cell lysate, 293T cell lysate, HeLa cell lysate. |
| Subcellular location: | Cytoplasm, Mitochondrion. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q05823 Human |
| Alternative names: | 2 5A dependent ribonuclease 2 5A dependent RNase 2' 5' oligoisoadenylate synthetase dependent 2-5A-dependent ribonuclease 2-5A-dependent RNase DKFZp781D08126 Interferon induced 2 5A dependent RNase MGC104972 MGC133329 PRCA 1 PRCA1 Ribonuclease 4 Ribonuclease L (2' 5' oligoisoadenylate synthetase dependent) Ribonuclease L Ribonuclease4 RibonucleaseL RN5A_HUMAN RNase L Rnasel RNS 4 RNS4 |
Images
|
Fig1:
Western blot analysis of RNase L on different lysates with Rabbit anti-RNase L antibody (HA722221) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 20ng/mL IFN-γ for 48 hours cell lysate Lane 3: 293T cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 75 kDa Exposure time: Lane 1-2: 6 seconds; Lane 3-4: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722221) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.