NSUN5 Recombinant Rabbit Monoclonal Antibody [PSH05-07]
cat.: HA722223
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH05-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human NSUN5 aa 51-100 / 429. |
| Positive control: | HeLa cell lysate, Caco-2 cell lysate, K-562 cell lysate, A431 cell lysate, MEF cell lysate, PC-12 cell lysate, human kidney tissue lysate, mouse kidney tissue lysate, mouse spleen tissue lysate, rat spleen tissue lysate. |
| Subcellular location: | Nucleus, nucleolus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q96P11 Human | Q8K4F6 Mouse Entrez Gene: 288595 Rat |
| Alternative names: | FLJ10267 MGC986 NOL1 NOL1-related protein NOL1/NOP2/Sun domain family member 5 NOL1R NOP2/Sun domain family, member 5 NOP2/Sun domain family, member 5A NSUN5 NSUN5_HUMAN p120 Putative methyltransferase NSUN5 WBSCR20 WBSCR20A Williams Beuren syndrome chromosome region 20A Williams-Beuren syndrome chromosomal region 20A protein Williams-Beuren syndrome critical region protein 20 copy A |
Images
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Fig1:
Western blot analysis of NSUN5 on different lysates with Rabbit anti-NSUN5 antibody (HA722223) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Caco-2 cell lysate Lane 3: K-562 cell lysate Lane 4: A431 cell lysate Lane 5: MEF cell lysate Lane 6: PC-12 cell lysate Lane 7: Human kidney tissue lysate Lane 8: Mouse kidney tissue lysate Lane 9: Mouse spleen tissue lysate Lane 10: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722223) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.