CARM1 Recombinant Rabbit Monoclonal Antibody [PSH05-08]
cat.: HA722224
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH05-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CARM1 aa 101-500 / 608. |
| Positive control: | MCF7 cell lysate, MDA-MB-231 cell lysate, A431 cell lysate, HEK-293 cell lysate, HCT 116 cell lysate, EA.hy926 cell lysate, NIH/3T3 cell lysate, mouse placenta tissue lysate, human testis tissue, human small intestine tissue, mouse small intestine tissue, rat small intestine tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Chromosome. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q86X55 Human | Q9WVG6 Mouse | Q4AE70 Rat |
| Alternative names: | carm1 CARM1_HUMAN Coactivator associated arginine methyltransferase 1 Coactivator-associated arginine methyltransferase 1 Histone arginine methyltransferase CARM 1 Histone arginine methyltransferase CARM1 Histone-arginine methyltransferase CARM1 PRMT 4 PRMT4 Protein arginine methyltransferase Protein arginine N methyltransferase 4 Protein arginine N-methyltransferase 4 |
Images
|
Fig1:
Western blot analysis of CARM1 on different lysates with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: MDA-MB-231 cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: HEK-293 cell lysate (20 µg/Lane) Lane 5: HCT 116 cell lysate (20 µg/Lane) Lane 6: EA.hy926 cell lysate (20 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Lane 8: Mouse placenta tissue lysate (40 µg/Lane) Predicted band size: 66 kDa Observed band size: 60 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722224) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722224) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722224) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722224) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-CARM1 antibody (HA722224) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722224) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
CARM1 was immunoprecipitated from 0.2 mg HCT 116 cell lysate with CARM1 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722224 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HCT 116 cell lysate (input) Lane 2: HA722224 IP in HCT 116 cell lysate Lane 3: Rabbit IgG instead of HA722224 in HCT 116 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 51 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.