RAC1 Recombinant Rabbit Monoclonal Antibody [PSH05-09]
cat.: HA722225
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: PSH05-09
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 21 kDa
Isotype: IgG
Immunogen: Recombinant protein within human RAC1 aa 1-192 / 192.
Positive control: HEK-293 cell lysate, Huh7 cell lysate, HUVEC cell lysate, HL-60 cell lysate, HeLa cell lysate, HL-60.
Subcellular location: Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus, Synapse.
Recommended Dilutions:
  WB
  IF-Cell

1:2,000
1:100
Uniprot #: SwissProt: P63000 Human
Alternative names: Cell migration inducing gene 5 protein Cell migration-inducing gene 5 protein MGC111543 MIG5 Migration inducing gene 5 Migration inducing protein 5 p21 Rac1 p21-Rac1 Rac 1 RAC1 RAC1_HUMAN Ras like protein TC25 Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) Ras-like protein TC25 Ras-related C3 botulinum toxin substrate 1 Rho family small GTP binding protein Rac1 TC 25 TC25
Images
HA722225_1.jpg Fig1: Western blot analysis of RAC1 on different lysates with Rabbit anti-RAC1 antibody (HA722225) at 1/2,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: Huh7 cell lysate
Lane 3: HUVEC cell lysate
Lane 4: HL-60 cell lysate
Lane 5: HeLa cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 21 kDa
Observed band size: 21 kDa

Exposure time: 2 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722225) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722225_2.jpg Fig2: Immunocytochemistry analysis of HL-60 cells labeling RAC1 with Rabbit anti-RAC1 antibody (HA722225) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RAC1 antibody (HA722225) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.