Wee1 Recombinant Rabbit Monoclonal Antibody [PSH05-11]
cat.: HA722227
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH05-11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 72 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Wee1 aa 251-600 / 646. |
| Positive control: | HeLa cell lysate, Raji cell lysate, 293T cell lysate, HT-29 cell lysate, Jurkat cell lysate, COS-1 cell lysate, human testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200 |
| Uniprot #: | SwissProt: P30291 Human |
| Alternative names: | DKFZp686I18166 EC 2.7.10.2 FLJ16446 MGC105683 OTTHUMP00000231338 OTTHUMP00000231339 Wee 1 WEE 1 homolog 1 (S. pombe) WEE1 WEE1 homolog (S. pombe) Wee1 homolog WEE1 homolog S. pombe Wee1 like protein kinase Wee1 tyrosine kinase Wee1+ homolog Wee1+ S. pombe homolog WEE1, S. pombe, homolog of WEE1, somatic Wee1-like protein kinase WEE1_HUMAN WEE1A Wee1A kinase WEE1hu |
Images
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Fig1:
Western blot analysis of Wee1 on different lysates with Rabbit anti-Wee1 antibody (HA722227) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: 293T cell lysate Lane 4: HT-29 cell lysate Lane 5: Jurkat cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 72 kDa Observed band size: 90 kDa Exposure time: 43 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722227) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Wee1 antibody (HA722227) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722227) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.