Fast Myosin Skeletal Heavy chain Recombinant Rabbit Monoclonal Antibody [PSH05-12]
cat.: HA722228
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, IF-Tissue, mIHC |
| Clonality: | Monoclonal |
| Clone number: | PSH05-12 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 223 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Fast Myosin Skeletal Heavy chain aa |
| Positive control: | Human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue. |
| Subcellular location: | Cytoplasm, myofibril. |
| Recommended Dilutions:
IHC-P IF-Tissue mIHC |
1:2,000-1:10,000 1:400-1:2,000 1:2,000 |
| Uniprot #: | SwissProt: P12882 Human | Q5SX40 Mouse Entrez Gene: 287408 Rat |
| Alternative names: | adult 1 MYH1 MYH1_HUMAN MyHC-2x MyHC-IIx/d Myosin heavy chain 1 Myosin heavy chain 2x Myosin heavy chain Myosin heavy chain IIx/d Myosin-1 skeletal muscle |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue / human brain tissue (negative) with Rabbit anti-Fast Myosin Skeletal Heavy chain antibody (HA722228) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722228) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue / mouse brain tissue (negative) with Rabbit anti-Fast Myosin Skeletal Heavy chain antibody (HA722228) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722228) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue / rat brain tissue (negative) with Rabbit anti-Fast Myosin Skeletal Heavy chain antibody (HA722228) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722228) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.