Strep-Tag II Recombinant Rabbit Monoclonal Antibody [PSH05-13]
cat.: HA722229
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH05-13 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Strep-tag II. |
| Recommended Dilutions:
WB IF-Cell IP |
1:10,000 1:2,500 1-2μg/sample |
| Alternative names: | anti-Strep-Tag II Strep-Tag II Strep-Tag II Strep-Tag IIantiboy |
Images
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Fig1:
Western blot analysis of Strep-Tag II on different lysates with Rabbit anti-Strep-Tag II antibody (HA722229) at 1/10,000 dilution. Lane 1: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate Lane 2: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722229) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling Strep-Tag II with Mouse anti-Strep-Tag II antibody (HA722229) at 1/2,500 dilution. HeLa cells, transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) or ACAT2 (N-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Strep-Tag II antibody (HA722229) at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Strep-Tag II was immunoprecipitated from 0.2 mg 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate with HA722229 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722229 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate (input) Lane 2: HA722229 IP in 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate Lane 3: Rabbit IgG instead of HA722229 in 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds; ECL: K1801 |
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Fig4:
Strep-Tag II was immunoprecipitated from 0.2 mg 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate with HA722229 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722229 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate (input) Lane 2: HA722229 IP in 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate Lane 3: Rabbit IgG instead of HA722229 in 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.