AHA1 Recombinant Rabbit Monoclonal Antibody [JE35-17]
cat.: HA722238
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE35-17 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human AHA1 aa 289-338 / 338. |
| Positive control: | HeLa cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, Endoplasmic reticulum. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O95433 Human | Q8BK64 Mouse Entrez Gene: 681996 Rat |
| Alternative names: | Activator of 90 kDa heat shock protein ATPase homolog 1 Activator of heat shock 90kDa protein ATPase homolog 1 AHA 1 AHA1 AHSA 1 Ahsa1 AHSA1_HUMAN C14orf3 HSPC322 p38 |
Images
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Fig1:
Western blot analysis of AHA1 on different lysates with Rabbit anti-AHA1 antibody (HA722238) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 42 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722238) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.