CRABP1 Recombinant Rabbit Monoclonal Antibody [JE40-53]
cat.: HA722241
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE40-53 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CRABP1 aa 88-137 / 137. |
| Positive control: | SH-SY5Y cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, e14.0 mouse embryonic brain tissue lysate, human breast cancer tissue, human thyroid tissue, mouse embryo tissue. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:200-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P29762 Human | P62965 Mouse |
| Alternative names: | Cellular retinoic acid binding protein 1 Cellular retinoic acid-binding protein 1 Cellular retinoic acid-binding protein I CRABP 1 CRABP CRABP I CRABP-I Crabp1 CRABPI RABP1_HUMAN RBP 5 RBP5 Retinoic acid binding protein I cellular |
Images
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Fig1:
Western blot analysis of CRABP1 on different lysates with Rabbit anti-CRABP1 antibody (HA722241) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: Caco-2 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: E14.0 mouse embryonic brain tissue lysate (40 µg/Lane) Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722241) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CRABP1 antibody (HA722241) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722241) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-CRABP1 antibody (HA722241) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722241) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-CRABP1 antibody (HA722241) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722241) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue with Rabbit anti-CRABP1 antibody (HA722241) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722241) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.