Phospho-TAK1 (S439) Recombinant Rabbit Monoclonal Antibody [JE44-93]
cat.: HA722242
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE44-93 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Ser439 of mouse TAK1. |
| Positive control: | HeLa cell lysate, HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, K-562 cell lysate, C2C12 cell lysate, C6 cell lysate, HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate, PC-12 cell lysate, PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB |
1:5,000-1:25,000 |
| Uniprot #: | SwissProt: O43318 Human | Q62073 Mouse | P0C8E4 Rat |
| Alternative names: | M3K7_HUMAN MAP3K 7 Map3k7 MEKK7 Mitogen activated protein kinase kinase kinase 7 Mitogen-activated protein kinase kinase kinase 7 TAK1 TGF beta activated kinase 1 TGF-beta-activated kinase 1 TGF1a Transforming growth factor beta activated kinase 1 Transforming growth factor-beta-activated kinase 1 |
Images
|
Fig1:
Western blot analysis of Phospho-TAK1 (S439) on different lysates with Rabbit anti-Phospho-TAK1 (S439) antibody (HA722242) at 1/25,000 dilution. Lane 1: K-562 cell lysate Lane 2: C2C12 cell lysate Lane 3: C6 cell lysate Lane 4: HeLa cell lysate Lane 5: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: NIH/3T3 starved overnight then treated with 100nM Calyculin A for 30 minutes cell lysate Lane 8: PC-12 cell lysate Lane 9: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722242) at 1/25,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-TAK1 (S439) on different lysates with Rabbit anti-Phospho-TAK1 (S439) antibody (HA722242) at 1/5,000 dilution and pan TAK1 antibody (ET1705-14) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 3: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722242) at 1/5,000 dilution and pan TAK1 antibody (ET1705-14) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.