Presenilin 2 / AD5 Recombinant Rabbit Monoclonal Antibody [PSH05-18]
cat.: HA722243
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH05-18 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Presenilin 2 aa 300-400. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, 293T cell lysate, SH-SY5Y cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat liver tissue lysate. |
| Subcellular location: | Endoplasmic reticulum membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
WB IP |
1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P49810 Human | Q61144 Mouse | O88777 Rat |
| Alternative names: | AD3L AD3LP AD4 AD5 Alzheimer disease 4 CMD1V E5-1 OTTHUMP00000035671 OTTHUMP00000035672 OTTHUMP00000228286 OTTHUMP00000228288 Presenilin 2 (Alzheimer disease 4) Presenilin 2 Presenilin-2 CTF subunit PS-2 PS2 Psen2 PSN2_HUMAN PSNL2 STM-2 STM2 |
Images
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Fig1:
Western blot analysis of Presenilin 2 / AD5 on different lysates with Rabbit anti-Presenilin 2 / AD5 antibody (HA722243) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (20 µg/Lane) Lane 5: SH-SY5Y cell lysate (20 µg/Lane) Lane 6: RAW264.7 cell lysate (20 µg/Lane) Lane 7: Neuro-2a cell lysate (20 µg/Lane) Lane 8: C2C12 cell lysate (20 µg/Lane) Lane 9: NIH/3T3 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Lane 11: C6 cell lysate (20 µg/Lane) Lane 12: Human brain tissue lysate (40 µg/Lane) Lane 13: Mouse brain tissue lysate (40 µg/Lane) Lane 14: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 23 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722243) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Presenilin 2 / AD5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722243 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722243 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA722243 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA722243 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.