CD169 Recombinant Rabbit Monoclonal Antibody [PSH05-21]
cat.: HA722246
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH05-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 183 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD169 aa 1-1,000 / 1,709. |
| Positive control: | K-562 cell lysate, RAW264.7 cell lysate, THP-1 cells treated with 50ng/mL PMA for 72 hours add 1μg/mL LPS for 24 hours, RAW264.7 cells treated with 1μg/mL LPS for 24 hours. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB FC |
1:2,000 1:2,000 |
| Uniprot #: | SwissProt: Q9BZZ2 Human | Q62230 Mouse |
| Alternative names: | CD169 CD169 antigen dJ1009E24.1 DKFZp667F058 FLJ00051 FLJ00055 FLJ00073 FLJ00411 FLJ32150 Sialic acid binding Ig like lectin 1 sialic acid binding Ig-like lectin 1, sialoadhesin Sialic acid binding immunoglobulin like lectin 1 Sialic acid-binding Ig-like lectin 1 Sialoadhesin Siglec 1 Siglec-1 Siglec1 SN SN_HUMAN |
Images
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Fig1:
Western blot analysis of CD169 on different lysates with Rabbit anti-CD169 antibody (HA722246) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 183 kDa Observed band size: 220 kDa Exposure time: 28 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722246) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of THP-1 cells (left) and THP-1 cells treated with 50ng/mL PMA for 72 hours add 1μg/mL LPS for 24 hours (right) labeling CD169. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722246, 0.5μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig3:
Flow cytometric analysis of RAW264.7 cells (left) and RAW264.7 cells treated with 1μg/mL LPS for 24 hours (right) labeling CD169. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722246, 0.5μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.