ICOS Recombinant Rabbit Monoclonal Antibody [JE39-99]
cat.: HA722247
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE39-99 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ICOS aa 1-100 / 199. |
| Positive control: | Mouse spleen tissue lysate, mouse thymus tissue lysate, mouse colon tissue lysate, rat spleen tissue lysate, rat thymus tissue lysate. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9WVS0 Mouse | Q9R1T7 Rat |
| Alternative names: | Activation inducible lymphocyte immunomediatory molecule Activation-inducible lymphocyte immunomediatory molecule AILIM CD278 CD278 antigen CD28-related Protein-1 CRP1 CVID1 ICOS ICOS_HUMAN Inducible costimulator Inducible T cell co stimulator Inducible T-cell costimulator MGC39850 |
Images
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Fig1:
Western blot analysis of ICOS on different lysates with Rabbit anti-ICOS antibody (HA722247) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse thymus tissue lysate Lane 3: Mouse colon tissue lysate Lane 4: Rat spleen tissue lysate Lane 5: Rat thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722247) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.