Phospho-GCN2 (T899) Recombinant Rabbit Monoclonal Antibody [JE00-51]
cat.: HA722249
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE00-51 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 187 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Thr899 of Human GCN2. |
| Positive control: | HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, Neuro-2a treated with 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q9P2K8 Human | Q9QZ05 Mouse Entrez Gene: 114859 Rat |
| Alternative names: | E2AK4_HUMAN Eif2ak4 Eukaryotic Translation Initiation Factor 2 alpha kinase 4 Eukaryotic translation initiation factor 2-alpha kinase 4 GCN2 GCN2 eIF2alpha kinase GCN2 like protein GCN2-like protein KIAA1338 MGCN2 |
Images
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Fig1:
Western blot analysis of Phospho-GCN2 (T899) on different lysates with Rabbit anti-Phospho-GCN2 (T899) antibody (HA722249) at 1/1,000 dilution and pan GCN2 antibody (ET1704-68) at 1/1,000 dilution. Lane 1: HeLa cell lysate (25 µg/Lane) Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate (25 µg/Lane) Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour (25 µg/Lane) Predicted band size: 187 kDa Observed band size: 187 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722249) at 1/1,000 dilution and pan GCN2 antibody (ET1704-68) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-GCN2 (T899) on different lysates with Rabbit anti-Phospho-GCN2 (T899) antibody (HA722249) at 1/2,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: Neuro-2a treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane) Lane 1: C6 cell lysate (20 µg/Lane) Lane 2: C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate (20 µg/Lane) Predicted band size: 187 kDa Observed band size: 187 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722249) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.