Munc18-1 Recombinant Rabbit Monoclonal Antibody [JE38-54]
cat.: HA722250
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE38-54 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Munc18-1 aa 401-500 / 594. |
| Positive control: | SH-SY5Y cell lysate, SiHa cell lysate, HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human testis tissue, human brain tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cytoplasm, cytosol, Membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000-1:5,000 1:200-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P61764 Human | O08599 Mouse | P61765 Rat |
| Alternative names: | FLJ37475 Munc 18 1 Munc 18a MUNC18 1 N-Sec1 Neuronal SEC1 NSec1 p67 Protein unc-18 homolog 1 Protein unc-18 homolog A Rb sec1 RBSEC1 STXB1_HUMAN STXBP1 Syntaxin binding protein 1 Syntaxin-binding protein 1 Unc 18 homolog Unc 18A Unc-18A Unc18 1 UNC18 Unc18-1 |
Images
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Fig1:
Western blot analysis of Munc18-1 on different lysates with Rabbit anti-Munc18-1 antibody (HA722250) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SiHa cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722250) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Munc18-1 on different lysates with Rabbit anti-Munc18-1 antibody (HA722250) at 1/5,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: MEF cell lysate Lane 3: C6 cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: Lane 1-3: 20 seconds; Lane 4-5: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722250) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Munc18-1 antibody (HA722250) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722250) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Munc18-1 antibody (HA722250) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722250) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Munc18-1 antibody (HA722250) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722250) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Munc18-1 antibody (HA722250) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722250) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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