ATG4C Recombinant Rabbit Monoclonal Antibody [PSH05-23]
cat.: HA722252
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH05-23 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ATG4C aa 1-458 / 458. |
| Positive control: | 293T cell lysate, Jurkat cell lysate, Raji cell lysate, A431 cell lysate, HeLa cell lysate, K-562 cell lysate, HL-60 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, COS-1 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q96DT6 Human | Q811C2 Mouse Entrez Gene: 313391 Rat |
| Alternative names: | APG4 autophagy 4 homolog C (S. cerevisiae) APG4 autophagy 4 homolog C APG4 C APG4-C APG4C ATG 4C ATG4 autophagy related 4 homolog C (S. cerevisiae) ATG4 autophagy related 4 homolog C Atg4c ATG4C_HUMAN AUT (S. cerevisiae) like 1, cysteine endopeptidase AUT like 1, cysteine endopeptidase (S. cerevisiae) AUT like 1, cysteine endopeptidase AUT like 3 cysteine endopeptidase AUT-like 3 cysteine endopeptidase AUTL1 AUTL3 Autophagin 3 Autophagin-3 Autophagy related 4C cysteine peptidase Autophagy related cysteine endopeptidase 3 Autophagy related protein 4 homolog C Autophagy-related cysteine endopeptidase 3 Autophagy-related protein 4 homolog C Cysteine protease ATG4C EC 3.4.22 FLJ14867 OTTHUMP00000010715 |
Images
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Fig1:
Western blot analysis of ATG4C on different lysates with Rabbit anti-ATG4C antibody (HA722252) at 1/2,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: Raji cell lysate (20 µg/Lane) Lane 4: A431 cell lysate (20 µg/Lane) Lane 5: HeLa cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (20 µg/Lane) Lane 7: HL-60 cell lysate (20 µg/Lane) Lane 8: Mouse brain tissue lysate (40 µg/Lane) Lane 9: Rat brain tissue lysate (40 µg/Lane) Lane 10: COS-1 cell lysate (20 µg/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722252) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
ATG4C was immunoprecipitated from 0.2 mg Raji cell lysate with HA722252 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722252 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input) Lane 2: HA722252 IP in Raji cell lysate Lane 3: Rabbit IgG instead of HA722252 in Raji cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 21 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.