DNA Polymerase lambda Recombinant Rabbit Monoclonal Antibody [PSH05-24]
cat.: HA722253
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH05-24 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human DNA Polymerase lambda aa 1-575 / 575. |
| Positive control: | HeLa cell lysate, A549 cell lysate, 293T cell lysate, HepG2 cell lysate, mouse testis tissue lysate, COS-1 cell lysate, human testis tissue. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q9UGP5 Human | Q9QXE2 Mouse |
| Alternative names: | BETA N BETAN DNA directed DNA polymerase lambda DNA polymerase beta 2 DNA polymerase beta-2 DNA polymerase kappa DNA polymerase kappa DNA polymerase beta N DNA polymerase lambda DNA polymerase lamda2 DPOLL_HUMAN EC 2.7.7.7,EC 4.2.99. FLJ46002 OTTHUMP00000020321 OTTHUMP00000020323 OTTHUMP00000059179 Pol beta2 POL KAPPA Pol Lambda POLKAPPA POLL Polymerase DNA directed lambda |
Images
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Fig1:
Western blot analysis of DNA Polymerase lambda on different lysates with Rabbit anti-DNA Polymerase lambda antibody (HA722253) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: Mouse testis tissue lysate (40 µg/Lane) Lane 6: COS-1 cell lysate (20 µg/Lane) Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 2 minutes 22 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722253) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DNA Polymerase lambda antibody (HA722253) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722253) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.