Human CD33 Recombinant Rabbit Monoclonal Antibody [PSH05-25]
cat.: HA722254
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH05-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD33 aa 18-259 (P20138). |
| Positive control: | Recombinant Human CD33 protein (HA210949). |
| Subcellular location: | Cell membrane; Peroxisome. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-26] to Human CD33 (Detector) (HA722255) and Recombinant Human CD33 protein (HA210949) as the standard. The reference range value is 22.6-5500pg/ml. |
| Uniprot #: | SwissProt: P20138 Human |
| Alternative names: | CD 33 CD33 CD33 antigen (gp67) CD33 antigen CD33 molecule CD33_HUMAN FLJ00391 gp67 My9 Myeloid cell surface antigen CD33 Myeloid cell surface antigen CD33 precursor Myeloid differentiation antigen CD33 p67 Sialic acid binding Ig like lectin 3 Sialic acid binding immunoglobulin like lectin 3 Sialic acid-binding Ig-like lectin 3 SIGLEC 3 Siglec-3 SIGLEC3 |
Images
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Fig1:
Sandwich ELISA analysis of Human CD33 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722254) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD33 protein (HA210949) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA722255, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: The concentrations of CD33 were interpolated from the CD33 standard curves and corrected for sample dilution. Undiluted samples are mixed serum from twenty-one volunteers 5%. The mean CD33 concentration was determined to be 7,466 pg/mL in mixed serum from twenty-one volunteers. |
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Fig3:
Interpolated concentrations of native CD33 in U937 and HL-60 extract samples based on a 1,000 µg/mL extract load. The concentrations of CD33 were measured in triplicates, interpolated from the CD33 standard curve and corrected for sample dilution. Undiluted samples are U937 extract 5% and HL-60 extract 1%. The mean CD33 concentration was determined to be 5,333 pg/mL in U937 extract and 21,225 pg/mL in HL-60 extract. |
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Fig4:
Interpolated concentrations of spiked CD33 in human cell culture media samples. The concentrations of CD33 were measured in triplicates, interpolated from the CD33 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.