Human CD200R1 Recombinant Rabbit Monoclonal Antibody [PSH05-27]
cat.: HA722256
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH05-27 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD200R1 aa 29-243 (Q8TD46). |
| Positive control: | Recombinant standard Human CD200R1 protein (HA210572). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-28] to Human CD200R1 (Detector) (HA722257) and recombinant standard Human CD200R1 protein (HA210572). The reference range value is 14.4-3500pg/ml. |
| Uniprot #: | SwissProt: Q8TD46 Human |
| Alternative names: | CD 200R CD200 cell surface glycoprotein receptor CD200 receptor 1 Cd200r1 Cell surface glycoprotein CD200 receptor 1 Cell surface glycoprotein OX2 receptor 1 Cell surface glycoprotein OX2 receptor cell surface glycoprotein receptor CD200 CRTR 2 CRTR2 HCRTR2 MO2R1_HUMAN MOX2 receptor MOX2R OX2R |
Images
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Fig1:
Sandwich ELISA analysis of Human CD200R1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722256) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD200R1 protein (HA210572) starting from 3,500 pg/ml to 0 pg/ml and detect antibody (HA722257, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: The concentrations of CD200R1 were interpolated from the CD200R1 standard curves and corrected for sample dilution.Undiluted samples are mixed serum from twenty-four volunteers 25%. The mean CD200R1 concentration was determined to be 3,702 pg/mL in mixed serum from twenty-four volunteers. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.