Human LAG-3 Recombinant Rabbit Monoclonal Antibody [PSH05-29]
cat.: HA722258
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH05-29 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human LAG-3 aa 23-450 (P18627). |
| Positive control: | Recombinant human LAG-3 protein (HA210934). |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-30] to Human LAG-3 (Detector) (HA722259) and recombinant Human LAG-3 protein (HA210934) as the standard. The reference range value is 20.6-5000pg/ml. |
| Uniprot #: | SwissProt: P18627 Human |
| Alternative names: | CD223 CD223 antigen FDC protein LAG-3 Lag3 LAG3_HUMAN Lymphocyte activating 3 Lymphocyte activation gene 3 Lymphocyte activation gene 3 protein Protein FDC |
Images
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Fig1:
Sandwich ELISA analysis of Human LAG3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722258) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant standard Human LAG-3 protein (HA210934) starting from 5000 pg/ml to 0 pg/ml and detect antibody (HA722259, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: Conditioned media was harvested after 48 hours. LAG3 was measured in 100% unstimulated and PHA-M stimulated human PBMC cell supernatant. The concentrations of LAG3 were interpolated from the LAG3 standard curves. The mean LAG3 concentration was determined to be 526 pg/mL in PHA-M stimulated human PBMC cell supernatant. There was no detectable signal in unstimulated supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.