TRIF Recombinant Rabbit Monoclonal Antibody [PSH05-39]
cat.: HA722268
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH05-39 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TRIF aa 351-650 / 712. |
| Positive control: | Raji cell lysate, Jurkat cell lysate, THP-1 cell lysate, HeLa cell lysate, K-562 cell lysate. |
| Subcellular location: | Cytoplasmic vesicle, autophagosome, Cytoplasm, cytosol, Mitochondrion. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q8IUC6 Human | Q80UF7 Mouse Entrez Gene: 363328 Rat |
| Alternative names: | IIAE6 MGC35334 MyD88 3 Proline rich vinculin and TIR domain containing protein B Proline-rich PRVTIRB Putative NF kappa B activating protein 502H Putative NF-kappa-B-activating protein 502H Putative NFkB activating protein TCAM1_HUMAN TICAM 1 TICAM-1 Ticam1 TIR domain containing adapter molecule 1 TIR domain containing adapter protein inducing IFN beta TIR domain containing adaptor inducing interferon beta TIR domain-containing adapter molecule 1 TIR domain-containing adapter protein inducing IFN-beta Toll interleukin 1 receptor domain containing adapter protein inducing interferon beta Toll like receptor adaptor molecule 1 Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta TRIF TRIF protein vinculin and TIR domain-containing protein B |
Images
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Fig1:
Western blot analysis of TRIF on different lysates with Rabbit anti-TRIF antibody (HA722268) at 1/2,000 dilution. Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate Lane 3: THP-1 cell lysate Lane 4: HeLa cell lysate Lane 5: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 76 kDa Observed band size: 100 kDa Exposure time: 1 minute 2 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722268) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.