HA722269

TrkC Recombinant Rabbit Monoclonal Antibody [PSH05-40]

cat.: HA722269

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P, FC, IF-Tissue
Clonality:	Monoclonal
Clone number:	PSH05-40

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 94 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human TrkC aa 1-429 / 839.
Positive control:	TT cell lysate, mouse brain tissue lysate, rat brain tissue lysate, TT, human brain tissue, mouse brain tissue, rat brain tissue.
Subcellular location:	Membrane.
Recommended Dilutions: WB IF-Cell IHC-P FC IF-Tissue	1:1,000 1:100 1:2,000 1:1,000 1:200-1:400
Uniprot #:	SwissProt: Q16288 Human \| Q6VNS1 Mouse \| Q03351 Rat
Alternative names:	EC 2.7.10.1 ETS related protein neurotrophic receptor tyrosine kinase fusion ETS related protein neurotrophic receptor tyrosine kinase fusion protein ETV6 NTRK3 fusion GP145 TrkC gp145(trkC) GP145-TrkC GP145TrkC Neurotrophic tyrosine kinase receptor type 3 Neurotrophin 3 receptor NT 3 growth factor receptor NT 3 growth factor receptor precursor NT 3 receptor NT-3 growth factor receptor Ntrk3 NTRK3_HUMAN OTTHUMP00000192915 TRK C Trk-C TRKC TrkC tyrosine kinase Tyrosine kinase receptor C

Images

Fig1: Western blot analysis of TrkC on different lysates with Rabbit anti-TrkC antibody (HA722269) at 1/1,000 dilution.

Lane 1: TT cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Lane 4: Mouse liver tissue lysate (negative) (40 µg/Lane)
Lane 5: Rat liver tissue lysate (negative) (40 µg/Lane)

Predicted band size: 94 kDa
Observed band size: 94-130 kDa

Exposure time: 2 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722269) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Immunocytochemistry analysis of TT cells labeling TrkC with Rabbit anti-TrkC antibody (HA722269) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TrkC antibody (HA722269) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

	Fig3: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TrkC antibody (HA722269) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722269) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-TrkC antibody (HA722269) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722269) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TrkC antibody (HA722269) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722269) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-TrkC antibody (HA722269) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722269) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Flow cytometric analysis of TT cells labeling TrkC.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA722269, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.