Phospho-Cdk1/2/3 (T14) Recombinant Rabbit Monoclonal Antibody [JE04-37]
cat.: HA722272
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE04-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Thr14 of human Cdk1 protein (P06493). |
| Positive control: | SiHa cell lysate, Caco-2 cell lysate, HeLa cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, human colon tissue, human liver tissue, human spleen tissue, mouse spleen tissue, rat spleen tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P06493 Human | P24941 Human | Q00526 Human | P11440 Mouse | P97377 Mouse | Q80YP0 Mouse | P39951 Rat | Q63699 Rat |
| Alternative names: | Cdc 2 Cdc2 CDC28A CDK 1 CDK1 CDK1_HUMAN CDKN1 CELL CYCLE CONTROLLER CDC2 Cell division control protein 2 Cell division control protein 2 homolog Cell division cycle 2 G1 to S and G2 to M Cell division protein kinase 1 Cell Divsion Cycle 2 Protein Cyclin Dependent Kinase 1 Cyclin-dependent kinase 1 DKFZp686L20222 MGC111195 p34 Cdk1 p34 protein kinase P34CDC2 Cdc2 related protein kinase cdc2-related protein kinase CDC28 CDC2A Cdk 2 CDK2 CDK2_HUMAN CDKN2 Cell devision kinase 2 Cell division protein kinase 2 Cyclin dependent kinase 2 cyclin dependent kinase 2-alpha Cyclin-dependent kinase 2 kinase Cdc2 MPF p33 protein kinase p33(CDK2) Cdk 3 Cdk3 CDK3_HUMAN CDKN3 Cell division kinase 3 Cell division protein kinase 3 Cyclin dependent kinase 3 Cyclin-dependent kinase 3 OTTHUMP00000206828 p36 |
Images
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Fig1:
Western blot analysis of Phospho-Cdk1/2/3 (T14) on different lysates with Rabbit anti-Phospho-Cdk1/2/3 (T14) antibody (HA722272) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: Caco-2 cell lysate Lane 3: HeLa cell lysate Lane 4: PC-12 cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 30 kDa Exposure time: 43 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722272) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue untreated / treated with λpp with Rabbit anti-Phospho-Cdk1/2/3 (T14) antibody (HA722272) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722272) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Phospho-Cdk1/2/3 (T14) antibody (HA722272) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722272) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Phospho-Cdk1/2/3 (T14) antibody (HA722272) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722272) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Phospho-Cdk1/2/3 (T14) antibody (HA722272) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722272) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Phospho-Cdk1/2/3 (T14) antibody (HA722272) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722272) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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