GPR56 Recombinant Rabbit Monoclonal Antibody [PSH05-43]
cat.: HA722273
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH05-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 78 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GPR56 aa 26-402. |
| Positive control: | A-172 cell lysate, U-87 MG cell lysate, T-47D cell lysate, human brain tissue lysate, human glioma tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q9Y653 Human |
| Alternative names: | 7 transmembrane protein with no EGF like N terminal domains 1 BFPP DKFZp781L1398 EGF TM7 like G protein coupled receptor 56 G-protein coupled receptor 56 GPR 56 Gpr56 GPR56_HUMAN Polymicrogyria bilateral frontoparietal Protein TM7XN1 TM7LN4 TM7XN1 TM7XN1 protein |
Images
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Fig1:
Western blot analysis of GPR56 on different lysates with Rabbit anti-GPR56 antibody (HA722273) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: A-172 cell lysate Lane 2: U-87 MG cell lysate Lane 3: T-47D cell lysate Lane 4: Human liver tissue lysate (negative) Lane 5: Human brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 78 kDa Observed band size: 55-70 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722273) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human glioma tissue with Rabbit anti-GPR56 antibody (HA722273) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722273) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.